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PRESOLICITATION NOTICE The U.S. Geological Survey (USGS) intends to issue a Request for Quotation (RFQ) for a sequencing facility to conduct de novo epigenetic methylation clock development for the Mojave Desert tortoise using whole-genome enzymatic methylation and 5-base workflow, including the preparation and sequencing of DNA samples for methylation analysis, delivery of sequencing data, and de novo epigenetic methylation clock development. The objective is to generate data and identify loci that will be used to age tortoises. The Government anticipates awarding a firm-fixed-price purchase order for these services. Service Contract Act (SCA) is applicable and the wage determination corresponding to the location of performance will apply. ACQUISITION PROCEDURES This acquisition will be conducted using the simplified procedures for commercial products and commercial services under RFO Part 12. In accordance with RFO 12.201-1(c)(2), for acquisitions at or below the Simplified Acquisition Threshold, the Contracting Officer may solicit quotations directly from suppliers. When using direct solicitation procedures, the Government will: 1. Post a presolicitation notice in the Governmentwide Point of Entry (GPE) in accordance with RFO Subpart 5.1, unless an exception applies; 2. Solicit quotations from at least three sources; and 3. Whenever practicable, solicit quotations from two sources not included in the previous solicitation. Accordingly, the Government intends to solicit quo

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